Chemiluminescence in activated human neutrophils

Chemiluminescence of asbestos-activated macrophages

Oxidation of methionine residues in proteins of activated human neutrophils.

A simple assay for the detection of 35S-labeled methionine sulfoxide residues in proteins is described. The assay, which is based on the ability of CNBr to react with methionine but not with methionine sulfoxide, requires the prelabeling of cellular proteins with [35S]methionine. The assay was used to study the extent of methionine oxidation in newly synthesized proteins of both activated and q...

Chemiluminescence response of human neutrophils to He-Ne laser irradiation (in vivo and in vitro)

He-Ne laser irradiation (0.01 -6 3/cm3) of the blood and neutrophile suspension in vitrowas shown to modulate reactive oxygen species (RDS ) product ion in healthy donors. Intravascular laser irradiation of the blood ( 5 mW, 30 minutes, daily) of the patients with chronic gastric ulcer during first 5 days resulted in increasing stimul-k-duced ROS production in patients with the low initial chem...

Application of neutrophils chemiluminescence test in medical diagnostics

The ability of lighting is a characteristic trait of alive organisms on the organ, tissue or cell level. It is often called as a ultra-weak luminescence or even ”cold lighting” [1, 2]. This phenomenon could appear in vivo spontaneously. The described situation is in need of the energy coming from some biochemic reactions, which product very active compounds as oxygenic and lipids radicals. All ...

Role of myeloperoxidase in intracellular and extracellular chemiluminescence of neutrophils.

Activated polymorphonuclear leucocytes (neutrophils) can generate both intracellular and extracellular luminol dependent chemiluminescence. As luminol dependent chemiluminescence largely measures the activity of the myeloperoxidase-H2O2 system, and as the extracellular activity of this enzyme may be responsible for the tissue damage associated with inflammatory conditions such as rheumatoid art...